The role of a mid - tier soa cache architecture 中间层soa缓冲体系结构的作用
There were 5 . 9 tip residues in cml . the purified cml was modified with n - bromosuccinimide ( nbs ) in hac - naac buffer containing 8mol / l urea or not 在含8mol l脲和不含脲的缓冲体系中,用n -溴代丁二酰亚胺( nbs )修饰cml的色氨酸残基。
Using ki , cscl and acr to probe the micro - environments of trp residues in dpl , the trp residue fluorescence of dpl was not quenched by ki and cscl , but 100 % quenched by acr 在含脲的体系中可修饰36个色氨酸残基;在不含脲的缓冲体系中可修饰12个色氨酸残基。
Wang shun , lin juanjuan et al . , kinetics and mechanism of the reaction between thiourea and iodate in unbuffered medium , science in china , ser . b , 2004 , 47 ( 6 ) , 480 王舜,高庆宇,王新红,林娟娟,亚氯酸盐-硫代硫酸盐非缓冲体系的复杂动力学,物理化学学报, 2003 , 19 ( 8 ) , 762
The trials of nr / r single - dimensional sds - page and nr / r two - dimensional sds - page proved that cml had no disulfide bond . edta inhibited the agglutinate activity of cml and a small part of cml recovered its agglutinate activity after removing the edta 在ph7 . 0 、 ph5 . 0的缓冲体系中,用edta去除cml分子中金属离子的实验表明edta对cml的凝血活性具有抑制作用。