重复 1.repeat; duplicate; reduplicate; iterate 他把那个词重复了几遍。 he repeated the word several times.2.repetition; reiteration; gemination; rerun; duplication; replication; palin-; pali- 避免不必要的重复 avoid needless duplication; 经过无数次的重复 after numerous repetitions; 任何历史现象都不会是简单的重复。 no historical phenomenon is a mere repetition of the past
2 . 4 preparation of yeast telomerase , the activity of telomerase was examined by the method of trap , polyacrylamide gel electrophoresis and silver staining 2 . 4酵母端粒酶的制备,端粒重复扩增法( trap ) 、聚丙烯酞胺凝胶电泳及硝酸银染色法检测端粒酶活性。
That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers , then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting , and deposited by ethanol ) . sequencing analysis of pcr product was done on 310 gene scan analysis ? . 1 . 2 dna sequencer to determine telomerase activity . notably , this method eliminated the restraining factors of taq dna polymerase , making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results 在kim等开发的端粒重复扩增分析法( trap )的基础上进行改进,即通过对端粒酶延伸ts寡核昔酸反应产物的精制,消除了pcr扩增中抑制taq酶活性的因素,从而减少了样品之间pcr扩增上的差异和假阴性现象的发生,提高了判断样品端粒酶阴、阳性的准确率和定量的准确性。