姐妹 1.(姐和妹) sisters 同胞姐妹 sister of the whole blood; full sister; 异父[母] 姐妹 half sister; 姐妹之情 the affection of elder and younger sisters; 这姐妹俩容貌极相像。 the two sisters are as like as two peas.2.(兄弟姐妹) brothers and sisters 你们姐妹几个? how many brothers and sisters do you have?; 姐妹城市 sister city relationship; twinning of cities; twin cities; sister cities; 姐妹船舶条款 sister ship clause; 姐妹公司 sister company; 姐妹省[州] sister provinces [states]
2 . 5 ul of 10 x reaction buffer , 1 . 5 ul 25mm mgcl2 , 0 . 3 ul lomm dntp , 0 . 5 ul taqdna polymerase ( 5 u / ul ) , and lul ( = 20ng ) of primer were used in per reaction . each reaction was overlaid with one drop of paraffin oil . the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec , annealed at 37 1 min , extended at iv b 72 for 2 min and repeated the cycle 45 times , at last , extended at 72 ' c for lomin 等( 1995 )利用rapd标记区分美国东部一杂交地带的蟋蟀的两个姐妹种, allonemobiusfasciatus和a . socius ,并于1998年使用rapd和异型酶标ic做出了这两种蟋蟀的基因连锁图;国内田英芳、郑哲民( 20of )首次将rapd技术运用于蟋蟀总科的分子系统学研究中,采用2种引物对7种蟋蟀进行了基困组dna多态性研究,并应用upg问a法构建树状图,椎测系统发生关系