串联 1.(为共同行动进行联系) establish ties with; establish relation with; make contacts with; link up; establish; contact:土改后, 他们串联了几户农民组织起了一个生产互助组。after land reform, they linked up several farmers and organized an agriculture mutual-aid team.2.[电工] (串连) series connection; series; connect in series; concatenation; cascade; in-line; install in series; series arrangement; tandem compound; tandem connection 同... 串联 in tandem with ..
独联体 [简] (独立国家联合体) the commonwealth of the independent states (cis)
It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession 初步认为grb - ast _ 3串联体基因适宜采用融合表达载体表达。
Is encoded by grb ~ ast7 . the expression analysis of the concatemer was proceeded in the e . coli pet expression system 本文主要工作就是构建grb - ast _ 7串联体,将构建后的串联体导入大肠杆菌进行表达分析。
Neuropeptide ast2 is encoded by grb - ast3 . pcr methods were tried to construct grb - ast3 concatemer . the expression analysis of the concatemer was proceeded in the e . coli pet expression system 本论文采用改进的pcr “自身模板-引物”法进行构建方法构建grb - ast _ 3串联体,并将拼接好的串联体转入大肠杆菌进行表达分析。
Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase . one concatemer of 360bp , 9 copies of grb - ast3 , were obtained from the first pair of primer and three ones of 515bp , 750bp , 792bp , from the second one 从第一对引物的拼接产物中克隆到一个360bp含有9个拷贝数的grb - ast _ 3的串联体,从第二对引物的拼接产物中克隆到三个分别为515 、 750 、 792bp的grb - ast _ 3的串联体。
In this method , high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification . after cloning and sequencing of the pcr products , one concatemer contains seventeen copies of grb ~ ast7 was attained . the grb - ast ? concatemer was cloned into pet22b expression vector 先用t4dnapolymerase构建成模板,再通过高保真dna聚合酶( pyrobesttmdnapolymerase ) pcr进行扩增,从中得到一个含有17个拷贝的grb - ast _ 7的串联体。
Grb - ast3 concatemers were respectively cloned into expression vector , pet21b , pet22b , pet30a , in certain insertion orientation and correct reading frame must be insured . after the reading frame was confirmed correct by sequencing , the recombinant plasmid was transformed into expression host e . coli bl21 ( de3 ) plyss 根据基因在克隆载体psk上的插入方向,按照正确的阅读框,将grb - ast _ 3串联体基因分别克隆在大肠杆菌pet21b 、 pet22b 、 pet30a三个不同的表达载体上。