明目上清丸 eyesight-improving and u er-heat-removing bolus; eyesight-improving and up-per-heat-removing bolus; eyesight-improving and upper-heat-removing bolus
牛黄上清丸 cow-bezoar bolus for clearing away heat of the u er part of the body; cow-bezoar bolus for clearing away heat of the upper part of the body
2n methods 1 ) sample preparation the supernatant of cultures were digested with identical volume lysis buffer at 100 for 15 min . dna was isolated for use as template 二、实验方法1 、标本处理取hcmv低传代分离株细胞培养上清液与等量裂解液混匀后,煮沸15分钟,提取dna ,作为扩增模板。
Apoptosis was identified by dna electrophoresis and flow cytometry . the results showed the supernatant containing hsblys induced a does and concentration - dependant increase in apoptosis cells Dna琼脂糖凝胶电泳和流式细胞仪结果显示, hsblys表达上清可诱导k562细胞凋亡,且具有剂量依赖效应。
After that we replaced the media with fresh complete media every two or three days . for each passage the cells were plated at about 3 x 10 / ml and grew to confluency 细胞达80 。 t融合后用0 ? 25胰蛋白酶37消化2 3分钟,用全培养液终止消化后, 1000rpm离心5分钟,弃上清后用全培养液重新悬浮细胞。
After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography , the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants 表达菌甲醇诱导发酵;发酵上清液经超滤、硫酸胺粗分级分离以及阴离于交换层析,纯化出ctla4胞外区蛋白。
The reference min - a strain of pseudorabies virus ( prv ) was reproduced by adaption to pk - 15 cell and genome dna was extracted from the harvested infected culture after ultrasonification and centrifugation as template 将收获的细胞经- 20和37反复3次冻融后,超声粉碎,低速离心收集上清液,再以超速离收获病毒。
The transformants are fermented and induced by methnol , and finally the expression amount of ple is about 450mg / ml , and the mw is 60kda or so , the activity of supernatant of fermented broth is 2 . 21u / ml 阳性转化子经发酵诱导, ple表达量达450mg l ,且纯度高,分子量在60kda左右,发酵液上清酶活力达2 . 21u ml 。
Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot 将离心收集到的菌体超声破壁,离心分离,收集上清液,进行离子交换层析和分子筛分离纯化。
The activity of chitinase was detected by method of pahbah . the recombinat strain of p . pastoris was cultured in the inducing medium . the expression of chitinase was not detected by activity assay and sds - page 毕赤酵母经甲醇培养基诱导表达,检测破菌后上清液中的酶活性,并经sds - page检测,未发现外源蛋白的表达。
The supernatant containing hsblys co - stimulated b cell proliferation highly with anti - igm by mtt analysis . flow cytometry results showed only in the function of costim lation , can b cell entered the s phase of cell cycle Mtt法知hsblys表达上清对b淋巴细胞具有共刺激作用;流式细胞仪分析显示只有在共刺激作用下, b细胞才进入s期。
When the ph of acid liquid and supernatants was adjusted to 7 , there was no influence on growth trend of salmonella . but the amounts of salmonella in different growth stage were lower than the corresponding stage of the controlled group 将酸液和上清液的ph调至7后,对沙门氏菌的生长趋势无影响,但各阶段菌数均低于对照组。