With the improved method of ctab , the genomic dna extracted form plum and other species close to p . mume in genetic relationship could be directly used in pcr amplification , with good results obtained 选用改良ctab法提取的梅及其近缘种的基因组dna可直接用于pcr扩增分析,且效果良好。
Cloning , sequencing and identifying two novel cdna fragments related to chordoma are described in the first part and expression of fhit gene in chordoma is in the second part Dd一pcr产物应用凝胶系统分离、扫描,切下差异条带,重新进行pcr扩增纯化,扩增体系、条件同dd一pcr 。将纯化的。
3 characters of its sequences in the cardamine pcr technique was used to amplify its and the product was directly to be sequenced with two ways sequencing primers on sequenator 3碎米属植物的ts序列特征采用pcr扩增,混合扩增产物在自动测序仪上双向引物直接测序,测定了碎米荞属( ca厂内。
The main results are as below . 1 . we extracted rna from narcissus , cloned the cdna sequence of chs gene by rt - pcr and analyzed the coding sequence of gene 主要结果如下: ( 1 )以即将开放的中国水仙的花蕾为材料,采用异硫氰酸胍-苯酚-氯仿法提取rna ,反转录成cdna后用特异引物pcr扩增出1
The predicted bands were amplified from both to and ti generation transgenic plants by pcr , indicating that the hygromycin resistance gene ( hpt ) and svde gene were both transferred into tobacco Pcr扩增潮霉素抗性基因hpt和svde基因结果显示,在‘转淬回植株t斤t ;代中都分别扩增出互okb和二
Department of physiology 8 4 . the c6 cells were cultured for 3 days , then total rna was extracted from the cultured cells . 5 . the cdna was synthesized from the above - mentioned total rna , then pcr was used 将上述总rna反转录成cdna ,在进行pcr扩增,以确定是否有ilzrinrna与itlltna的表达: 6
( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed ( 2 )在翻译水平上通过pcr扩增的方式在t - pa基因起始密码子处添加了植物翻译起始共有序列aaca ,构建了植物表达载体pbet 。
The two genes were in an open reading frame . the fragment of pro3a + crylac10 without terminated structure was inserted into plasmid pbmb1205 when it was digested by bamhi and sphi 首先pcr扩增去掉终止结构的pro3a + cry ac10片段,用bamh 、 sph酶切pcr产物插入到解离载体pbmb1205上得到中间载体pbmb1205ac 。
2 ) screening using pcr . sequencing of pgex - hbrp the plasmid was screened by pcr , and 1 % agarose gel electrophoresis . the results show that there was one band at the respected sites about 750bp 2 ) pcr鉴定经过双酶切鉴定的质粒dna用pcr扩增,结果可见,在750bp处出现明显的带,表明插人的片段确是外源
A 358 bp pol ft cdna fragment framed with introduced restrict sites of xho i ( upstream ) and nhe i ( downstream ) was amplified by the method of rt - pcr , and then cloned into a ta - vector Rt一pcr扩增得到358hp的pcilpcdna片段,并在其上下游引物中分别引人了h 。 i和ie内切酶位点。 l此片段克隆人ta克隆载体后,经h 。