The fragments of gapdh gene and the potassium channel gene were amplified using the genomic dna of the four animals as a template 以基因组dna为模板,进行pcr扩增。扩增gapdh基因片段的大小约为300bp ,扩增钾通道基因片段的大小约为940bp 。
Search the snps in wnk4 genomic sequences design the primers to get the unique pcr products of wnk4 gene which cover the full length genomic sequences Dna的pgem一t载体为模板,用含有ha标签及酶切位点的上下游引物进行pcr扩增,酶切pedna3
When plants were grown , dna were extracted and transgenic lettuce plants were identified by pcr amplifying . seeds of transgenic plants were obtained . 4 本论文中获得的再生生菜植株通过gus染色和pcr扩增,证明导入了外源mbrazzein基因。
2 . ginlfyand ginndly gene were obtained from the leaf genomic dna of the male tree of ginkgo cultivar dafushou in the mid - april through pcr Dafushou )雄株基因组dna为模板,通过pcr扩增,获得两个银杏雄株leafy同源基因ginlfy和ginndly基因。
Detection of transgenic plants . the pcr assay of kan - resistant plants showed that the target gene had been integrated into tobacco accompanying with npt ii gene 转基因pcr检测pcr扩增npt11基因,证明thaumatin基因己导入烟草中,转化率为引
4 . genomic dna was extracted from some animal products provided by nanjing customs . partial mitochrondrial dna cytochrome b ( cyt b ) gene was amplified and sequenced 对南京海关送检的部分动物产品进行基因组dna提取, mtdnacytb基因的中文摘要pcr扩增和序列测定。
Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification 通过pcr扩增表明,转基因植株中可以检测出有目的基因( ipt基因)的存在,目的基因已成功地插入菊花的基因组中。
Through in situ hybridization and colony pcr , a positive recombinant plasmid was isolated from the genomic library and sequenced . the inserted dna fragment was about 4 . 3 kb 将pcr扩增片段用地高辛标记探针,利用原位杂交和菌落pcr从基因文库中获得含有阳性信号的重组质粒。
On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7 设计具有ndei和saci单限制性酶切位点的vp7基因引物,通过pcr扩增出vp7基因并经测序验证。
Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed . 3 通过合成六条长链引物,经两次pcr扩增,获得了238bp的植物防御素afp基因全长,并构建了植物表达载体peafp 。