pcr引物造句

例句与造句

1. According to the nucleotide sequence of selected antigen epitope , pcr primers supplemented with the ecor i and sal i sites were designed
2. Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated
3. The results indicated that the pcr primers designed could distinguish between marine bacteria and terrestrial bacteria , and could be applied in distinguishing the psychrophiles . sixteen strains which could produce cold - active protease and chitinase were screened by selective medium and
4. Compared with the referred rab3a , the amplified rab3a beard five nonsense nucleotide mutations , but the deduced amino acid sequence from the nucleotide sequence were completely the same as the referred rab3a protein , which demonstrated that the cloned placenta rab3a was suitable for further study
5. Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3
6. 用pcr引物造句挺难的，這是一个万能造句的方法
7. It encodes a protein of 865 amino acids with a signal peptide at the n - terminus , of which a polycystic - kidney - disease ( pkd ) - like domain , a triosephosphate - isomerase ( tim ) catalytic region , a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus
8. The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos . all of the eight segments were amplified by rt - pcr , and the purified pcr products were done cycle sequencing with specific primers , furthermore , the sequencing products were purified and run gel on abi prism 377 dna sequencing machine . the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4 . 0 version ) and genedoc ( 2 . 3 version ) software
9. And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
10. In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli