- cell cycle of hela cells
- hela
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细胞的细胞周期的英文
例句与用法
- Research result showed that the anticancer mechanisms of allicin were oxidation resistance , inhibiting the form of carcinogens , inducing tumor cello apoptosis , improving cellular immune function and enhancing radiosensitizing effect of antitumor drug
目前的研究结果显示，大蒜素的抑癌机制为：抗氧化，抑制致癌物的形成，影响肿瘤细胞的细胞周期并诱导肿瘤细胞调亡，增强机体免疫功能，对抗肿瘤药物有增敏作用等。 - Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具体方法为： ( 1 )照射后12h ，收集nih3t3细胞，用流式细胞仪检测各组细胞的细胞周期， pcr - sscp检测抑癌基因p16的变化； ( 2 ) nih3t3细胞照射后立即收集细胞和细胞上清，用试剂盒测量mda含量和sod 、 gsh - px的活性并观察其变化； ( 3 ) western免疫印迹和点杂交法检测照射2h后的各组hela细胞中基质金属蛋白酶- 2 ( mmp - 2 )的表达变化。