The studies and observations of effect inactivated vaccine canine distemper 犬瘟热灭活苗的研制及其预防效果观察
The hemolytic activity was optimally assayed at 20 ? , at ph 7 . 5 , and in the presence of 10 mm mg2 + 溶血活性是二价离子依赖的,热敏感的(溶血活性热灭活温度是45 ) 。
To compare the complement lytic system between cephalochordate and cartilaginous fish , lip shark ( chiloscyllium plagiosum ) serum complement activities were investigated 溶血活性是二价离子依赖的,热敏感的(溶血活性热灭活温度是45 ) 。
Irradiation or heat treatment protoplasts fusion were developed . equal protoplast suspensions ( containing about 108 / ml protoplasts ) of each strain were mixed and washed by centrifugation , then the pellets were resuspended in peg4000 solution ( 35 % ) at 37 ? for 2min . after centrifugation and resuspension , protoplasts were diluted and plated on regeneration medium 其次,确定了原生质体融合的基本条件及灭活原生质体融合的条件:等量混合两亲株原生质体或用热灭活(或紫外灭活)的原生质体( 10 ~ 8个ml ) ,用35的peg4000在37处理2min后,稀释,涂布再生,可获得融合率在50左右的融合子。