To establish a simple , inexpensive , rapid technology to detect point mutation or snp of mbl gene with high sensitivity and specificity , which should be of utility in the most of medical laboratories in our country 建立敏感性高、特异性强、简单快捷、适合我国国情(条件较差)的mbl基因点突变和snp的检测技术。
Mbl deficiency and low levels of serum mbl are strongly associated with the presence of variant mbl genes , encoding three different structural variants of the mbl polypeptide , which are caused by three point mutations , cgt52tgt , ggc54gac and gga57gaa , of mbl gene 已发现3个可引起免疫缺损的mbl结构基因点突变? ? cgt52tgt 、 ggc54gac和gga57gaa ,其编码产物氨基酸均发生改变,可导致血清mbl水平低下。
And pcr - rflp analysis was a sensitive , rapid and simple assay for the detection of nucleotide sequence change in ser 83 . and our data sugget that pcr - rflp may be a useful device for detecting the mutation in gyra gene associated the resistance to fqns 本研究通过试验条件的筛选,建立了pcr - rflp检测猪源致病性沙门氏菌gyra基因点突变的方法,可用于沙门氏菌氟喹诺酮类耐药性的分子水平检测和流行病学监测。
基因: gen; gene; Mendelian factor; ...点: drop突变: sudden change; change sudden ...单基因突变,点突变: single gene mutation操纵基因零点突变: operator zero点突变: point mutation操纵基因零式突变,操纵基因零点突变: operator zero mutation定点突变: rite-directed mutagenesis; site-directed mutagenesis; site-directed mutation零点突变: zero point mutation; zero-point mutation零点突变型: zero point mutant起点突变: zero point mutation; zeropoint mutation认可点突变: accepted point mutation受点突变: accepted point mutation随机点突变: random point mutation真点突变: true point mutation; truepoint mutation终点突变: end point mutation沉默位点突变: silent site mutation第二位点突变: second-site mutation特定位点突变: specific locus mutation矮胖亚位点突变率: dumpy sub-loci mutation rate点突变修复机制: point mutation repair mechanisms单基因突变: single gene mutation反突变基因: antimutator gene非基因突变: extragenic mutation基因内突变: intragenic mutation; intragenicmutation