The proliferation of cortical - hippocampal neurons was increased before 3 days and decreased after the fourth day when a 可不同程度地增加神经元数量,减少台盼蓝染色细胞数和
Cells in concentration - dependent manner . it was also found that the number of death cells stained by trypan - blue was increased by the addition of a ,细胞数量减少,部分细胞突起消失台盼蓝着色细胞数增加
After repeated adherence and enzyme digestion , the cells attained the requirement of morphological purification with a purity of more than 95 % 经台盼蓝拒染计数,将细胞按1 10 ^ 6接种于培养瓶,传代培养。
Use trypan blue exclusion method and mtt colorimetric method to detect u 2 - os cell growth inhibition caused by treatment of bfgf . 2 采用台盼蓝排除检测法和mth比色法检测bfgf对u2 os细胞生长的抑制情况。
After transfecting the shrna based on telomerase htert into hela cells with calcium phosphate co - precipitation procedures , we detected hela cells viability by trpan blue staining method 以磷酸钙共沉淀转染法将shrna转染hela细胞。于不同的时间用台盼蓝染色法检测细胞生存能力。