- double strand dna
- double stranded dna
- double-stranded dna
- ds dna
- dsdna
- duplex dna
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双链dna的英文
例句与用法
- Group i : dsdna viruses
双链dna病毒 - To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。 - Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。 - In order to study the expression of 3 - defensins in liver as acute phase response proteins , a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study . the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps . the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探针，经[ - ~ ( 32 ) p ] atp标记后通过northernblot方法检测mbd3在肝脏中的表达，同时分析了mbd3基因诱导表达的组织特异性，剂效和时效关系；结合mbd3基因启动子区序列分析，以- 164 ? - 179bp双链dna序列合成探针，经[ - ~ ( 32 ) p ] dctp标记后通过电泳迁移率改变实验( emsa )和south ? westernblot方法对参与mbd3在肝脏中诱导表达调节的转录因子进行分析。