The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation , the specificity of the antibody and the choice of a suitable enzyme label 酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用,其灵敏度取决于标记酶的高度纯化和高转化率。
A competitive binding assay used to determine hapten iaa with the aid of hrp - iaa conjugate has been developed . it was based on the ordered , stable mercapto self - assembled monolayer formed on gold surface , which immobilized igg with covalent bond 方法基于金基底上形成的均一、稳定、有序的巯基乙酸自组装单层膜,在edc 、 nhs活化下,能以共价方式固定抗体。
The protein accounting for the total was approximately 11 . 80 % . the peptide was synthesized according to the sequence of grb - ast7 . since ast7 is a hapten , it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc ) 根据ast _ 7氨基酸序列合成小肽,用edc做偶联剂把合成的半抗原小肽与载体bsa偶联成完全抗原,免疫家兔三次后,采血收集抗血清,用elisa测定效价为1 400 。
A hapten is a small molecule that can elicit an immune response only when attached to a large carrier such as a protein; the carrier may be one that also does not elicit an immune response by itself. (In general, only large molecules, infectious agents, or insoluble foreign matter can elicit an immune response in the body.
haptenとは意味:{名} : ハプテン hapten meaning: /hapˈtən/ (immunology) noun A substance, usu a small molecule, that can combine with an antibody but can initiate an immune response only when it is attached to a carrier mo...hapten en francais:n. haptène, espèce d'antigènehapten artinya:haptenhapten 뜻:noun, 합텐, 부착소(생체에 면역 반을을 일으키는 물질), =haptenhapten перевод:биол. гаптен, неполноценный антиген