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共转的英文

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  1. To investigate the consequence of this interaction , aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector . confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus
    在构建了红色荧光蛋白aes表达载体后,将其与tle绿色荧尤蛋白载体共转染细胞,共聚焦显微镜观察发现这两种分子在胞浆中有共存现象,而且aes的表达可抑制tlei向胞核内的聚积。
  2. In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
    本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。
  3. Finally , a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv . the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes . based on the expression of lacz gene , recombinant prv were selected and purified by blue - colored virus plaque
    利用脂质体介导的方法,将pltk - ha与prvbartha - k61基因组共转染于亚单层vero细胞,依据报告基因lacz在细胞中的表达,筛选蓝色蚀斑的重组病毒,经数次蚀斑纯化后, pcr鉴定、 western - blot分析。
  4. They are combination of co - transfection with rhe and gfp expressing plasmids , co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time . whichever the combination may be , we always observed the phenomena of chromatin bridge in co - transfected cells . these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits
    这时,存在三种可能的情况,即共转染rhe和pedna3 . 1 * kc 、共转染rhc和pedna3 . 1 + kg 、共转染rhe和rhc和pc洲a3 . 1 + kg ,但都观察到明显的染色质桥现象,进一步证明这种表型是人集缩素中smc亚基的缺陷的特征表型。

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相邻词汇

  1. "共注塑装置"英文
  2. "共祝圣餐"英文
  3. "共祝圣餐者"英文
  4. "共驻主存的"英文
  5. "共驻主存模块"英文
  6. "共转半径"英文
  7. "共转变"英文
  8. "共转导"英文
  9. "共转化"英文
  10. "共转化:同时转化两个细菌的标记基因。"英文
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